Anti-GFP （D5.1） XP antibody

Specificity / Sensitivity

GFP （D5.1） XP^® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

Western Blotting

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP^® Rabbit mAb.

IHC-P （paraffin）

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP^® Rabbit mAb.

Flow Cytometry

Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP^® Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP^® Rabbit mAb （red）. Blue pseudocolor = DRAQ5^® #4084 （fluorescent DNA dye）.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.

1. Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.

Application References

• Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.