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详细介绍
Overview
Product name
Anti-Mannose Receptor antibody [5C11]
See all Mannose Receptor products （6） ...
Description
Mouse monoclonal [5C11] to Mannose Receptor
Tested applications
WB, IHC-P, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human, Monkey
Immunogen
Recombinant Human Mannose Receptor protein （NP_002429.1）.
Positive control
Human lung （macrophages） tissue; Human pancreas lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.20
Constituent: 99% PBS
Concentration
- 50 µg at 0.5mg/ml
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5C11
Isotype
IgG1
Light chain type
kappa
Research areas
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
- Datasheet PDF
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Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-Mannose Receptor antibody [5C11] （ab117644）
![Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-Mannose Receptor antibody [5C11] （ab117644） image](https://img79.huajx.com/bb33f2c9b6dc3b859d91a8b6dfade9e95a5149f796166974f51a4c3d8c5815da516f957ac1cfadad.jpg)
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Western blot - Anti-Mannose Receptor antibody [5C11] （ab117644）
![Western blot - Anti-Mannose Receptor antibody [5C11] （ab117644） image](https://img77.huajx.com/bb33f2c9b6dc3b859d91a8b6dfade9e9a61e7511ca54f99f5bd26c7431012735400ea6a1d5db5137.jpg)
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Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] （ab117644）
![Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] （ab117644） image](https://img77.huajx.com/bb33f2c9b6dc3b859d91a8b6dfade9e9923ae3a46592afa28817a1e656387b6c29fd565db376a4f2.jpg)
（enlarge）
Applications
Show applications key
Our Abpromise guarantee covers the use of ab117644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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WB
WB: 1/500 - 1/1000. Pred...Read more →WB: 1/500 - 1/1000. Predicted molecular weight: 166 kDa.
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IHC-P
（1 Abreview）IHC-P: Use a concentrati...Read more →IHC-P: Use a concentration of 5 µg/ml.
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Sandwich ELISA
sELISA: Use at an assay ...Read more →sELISA: Use at an assay dependent concentration.
Target
Function
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Sequence similarities
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
Cellular localization
Membrane.
Information by UniProt
Alternative names
- C-type lectin domain family 13 member D antibody
- CD 206 antibody
- CD206 antibody
see all
Database links
- Entrez Gene: 4360 Human
- Omim: 153618 Human
- SwissProt: P22897 Human
- Unigene: 75182 Human
Anti-Mannose Receptor antibody [5C11] images:
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-Mannose Receptor antibody [5C11] （ab117644）
ab117644, at 5 µg/ml, staining Mannose Receptor in Formalin-fixed, Paraffin-embedded Human Lung （Macrophages） tissue by Immunohistochemistry.
Western blot - Anti-Mannose Receptor antibody [5C11] （ab117644）
Anti-Mannose Receptor antibody [5C11] （ab117644） + Human pancreas lysate
Predicted band size : 166 kDa
Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] （ab117644）
Detection limit for recombinant tagged Mannose Receptor is approximay 0.03ng/ml as a capture antibody.
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-Mannose Receptor antibody [5C11] （ab117644）
![Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-Mannose Receptor antibody [5C11] （ab117644）](https://img77.huajx.com/bb33f2c9b6dc3b859d91a8b6dfade9e9f5eab3666b75b858c93c7f217726a82a1738692822555e5e.jpg)
ab117644 staining Mannose Receptor in rhesus monkey brain tissue sections by Immunohistochemistry （IHC-P - paraformaldehyde-fixed, paraffin-embedded sections）.
Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody （1/200 in 10% serum in 20% Normal Horse Serum in 1X TTBS） for 12 hours at 37°C. A biotin-conjugated horse anti-mouse IgG polyclonal （1/200） was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Mr Peter Sullivan
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