详细介绍
促肾上腺皮质激素释放因子(CRF)检测试剂盒Human corticotropin-releasing factor,CRF 本试剂盒仅供研究使用检测范围:欢迎或或索取原版说明书zui低检测限:欢迎或或索取原版说明书,全国:欢迎选购!
促肾上腺皮质激素释放因子(CRF)检测试剂盒有效期:6个月
预期应用:ELISA法定量测定血清、血浆、细胞培养上清或其它相关生物液体中的含量.
说明
1.试剂盒保存:-20℃(较长时间不用时);2-8℃(频繁使用时).
2.浓洗涤液低温保存会有盐析出,稀释时可在水浴中加温助溶.
3.中、英文说明书可能会有不*之处,请以英文说明书为准.
4.刚开启的酶联板孔中可能会含有少许水样物质,此为正常现象,不会对实验结果造成任何影响.
实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗该指标抗体的微孔中依次加入标本或标准品、*化的抗该指标抗体、HRP标记的亲和素,经过*洗涤后用底物TMB显色.TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成zui终的黄色.颜色的深浅和样品中的该指标呈正相关.用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度.
试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔).
2. 标准品(Standard):2瓶(冻干品).
3. 样品稀释液(Sample Diluent):1×20ml/瓶.
4. *标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶.
6. *标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍.
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4).
10、标本的采集及保存
1. 血清:全血标本请于室温放置2小时或4℃隔夜后于1000 x g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
2. 血浆:可用EDTA或肝素作为抗凝剂,标本采集后30分钟内于2 - 8° C 1000 x g离心15分钟,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
3. 细胞培养物上清或其它生物标本:1000 x g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
注:标本溶血会影响zui后检测结果,因此溶血标本不宜进行此项检测.
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1062737 FastLane Cell cDNA Buffer Set (14x250ml)
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9019733 Motor, Z, V2, QX
9019726 Transport lock, V2, QX
9013404 Cable set, cooling system, 200
9238803 Rect. sealing cap, clear, incl. clips
9019653 Base Plate VPT, PyroQ96VW
9019626 Tip ejector, Cx2
9019603 Pins, locating, Mix and Reagent set, CG1
9019598 Lead, vac trap sensor, CX1
9019573 Belt, X-axis assembly, CQx
9019546 Clamp, cable, 3 wires 2 tabs, Cxx
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9019416 Box, shipping with foam packing, RG3
9019401 Connector, COM incl. int. cable, RG3
9019360 Switch, mains voltage selector, RG6
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9019339 LED, green (530), RG6
9019331 EPROM, IC Motherbrd, state FW, RG6
9019237 Linear guide - X, PyroQ24
9234673 X-Assembly Turntable Card
9234659 Cassette, HSPS, click'n'go
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9233630 Mousepad BR 01/10 (WW)
9019209 Motor - Gripper, PyroQ96MD
9019197 Damper kit - Robot, PyroQ96MD
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1062791 HBV PCR REAGENT III
9232903 Spritzenpumpe mMBP 2021C P7xxx
1061065 PG Influenza A RT-PCR Assay (48) Z
9232900 Valve, release, auto vac, BR8000
9016710 Software Package LiquiChip Analyzer
9019891 Pipetting head, 8 ch. , CX1
9019881 Lid with LED, red, RG6
9019857 36-well High Profile Locking Ring
9019837 IPC Hard Drive PCA6751, PyroQ96MD
9022832 Notebook_V3 W7_32bit
9013703 Tubing, syringe to connector, BR3000
9013485 Membrane, Simritz (20)
9238805 Round sealing cap, clear
9019637 Cable, UV lamp volt. conv., long, EZ1xl
9019590 Cover, plexiglas, 8 ch., CX1
9019565 Clamp, cable 2 wires 2 tabs, Cxx
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